Hi Manami, I think this is too little information to be able to provide meaningful comments.. Can you please send me models that you compare, and also tell which one you think is better and why? Also, send the data so that I can see overall picture. Then I might be able to say more. Pavel On 11/8/18 21:10, Manami Torimoto wrote:

Dear all, By phenix.refine, I attempted  to refine one molecule of protein. R-free was improved in certain wxc by setting the weight wxu manually, however, the results of “Temp. fact. variance analysis” in “Validation” tool of COOT was worsened. Why did such a thing happen?

Thank you.

Manami

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