Macromolecule structure refinements require geometry restraints including bonds, angles, dihedrals, chiral centres and planes. The restraints have been determined for protein residues and DNA/RNA. However, for many of the molecules routinely found in a protein crystal the restraints are either limited or unknown.

The electronic Ligand Builder and Optimisation Workbench (eLBOW) will generate restraints for known or novel ligands from a variety of inputs. This talk will cover using eLBOW to supply restraints for your structure refinements. Editing of the restraints using the Restraint Editor, Exclusively Ligands (REEL) will also be covered.