<div dir="ltr">Hi Luca,<div><br></div><div>Can you please verify if you have the "use starting model as reference" checkbox checked? You can find it if you click the "Options" button next to the "Reference model restraints" checkbox. If so, unchecking it should solve the problem.</div><div><br></div><div>Please let us know if you have any questions.</div><div><br></div><div>Best regards,</div><div>Oleg Sobolev.</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Dec 6, 2023 at 11:57 AM Luca Pellegrini <<a href="mailto:lp212@cam.ac.uk">lp212@cam.ac.uk</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
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<div>I am trying to use a reference model in real_space_refinement, but phenix stops with the message: “Cannot not restrain working model to self and a reference model simultaneously” (see below). The reference and working models are identical but
bound to different ligands. As far as a I can see, all I am doing is adding the reference model in the input window and ticking the box for reference model restraints (phenix 1.21rc1-4985).</div>
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<div>Best wishes,</div>
<div>Luca</div>
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<div>Luca Pellegrini, PhD<br>
Department of Biochemistry<br>
University of Cambridge<br>
Cambridge CB2 1GA<br>
UK</div>
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