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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal>Dear all,<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I am trying to build the model with Alphafold2 using Dock and Rebuild and encountered some issue with rebuild_predicted_model. It does not rebuild the model telling that: “Unable to morph model...may not match exactly in chain ID or residue numbers. No morphed model obtained... No docked model to work with.”<o:p></o:p></p><p class=MsoNormal>I use the model predicted with Alphafold2 google colab and the cryoem map with 3.5A resolution boxed around the protein of interest. The process_predicted_model and dock_predicted_model run successfully, giving the single-chain model which nicely fits into the map (40 of 300 residues are excluded due to low LDDT). The chain ID in the docked model is indeed different from predicted model: the predicted model has one chain A, but the docked one has A1. However, manually changing the chain ID either in the predicted or in the docked model does not resolve the issue. Manual runs of rebuild_predicted_model also fail with the same result.<o:p></o:p></p><p class=MsoNormal>Tried it with models and maps for different proteins, tried both on Linux and Windows – it gives the same output (Phenix 1.20.1.-4487). Please, find the log attached. I am seeking for any advice on resolving this problem. <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal style='margin-bottom:10.0pt;line-height:115%'>Best regards<br>Andrey Moiseenko<o:p></o:p></p><p class=MsoNormal style='margin-bottom:10.0pt;line-height:115%'>Electron Microscopy Lab @ Biology Department<br>Moscow State University<br><a href="mailto:postmoiseenko@gmail.com"><span style='color:blue'>postmoiseenko@gmail.com</span></a> <br>skype: callmoiseenko<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>