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<p class="MsoNormal">Hi all,</p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I had a question about comparing structures based on RMSD comparisons vs e<sup>-</sup>/A^3. While the former seems to be more frequently used in structural studies with a standard value of 1.0 rmsd for 2Fo-Fc and 3.0 rmsd for Fo-Fc, there
are differences in RMSD based on resolution. Hence, I wanted to ask if e<sup>-</sup>/A^3 could be a more appropriate comparison for looking at differences in electron density in a ligand-binding site between 2 structures of the same enzyme? So, here you would
try and set e<sup>-</sup>/A^3 of all the maps (2Fo-Fc and Fo-Fc) to the same value.</p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">The structures I�m referring to are between 2-2.5 angstroms in resolution.</p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I have also worked with the map sigma level comparison tool on PHENIX and wanted to know if that might be an alternate way to compare maps in 2 structures accordingly.
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Regards,</p>
<p class="MsoNormal">Vatsal</p>
<p class="MsoNormal"><o:p> </o:p></p>
<p style="margin:0cm;background:white"><span style="font-size:10.0pt;color:black">Post-doctoral fellow, Davis Lab, Emory University</span><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p></o:p></span></p>
<p style="margin:0cm;background:white"><span style="color:black"><a href="mailto:vatsal.purohit@emory.edu" target="_blank"><span style="font-size:10.0pt">vatsal.purohit@emory.edu </span></a></span><span style="font-size:10.0pt;color:black">| 346-719-9409</span><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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