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Hi Mohamed,<br>
<br>
perhaps you can copy some images from here:<br>
<br>
<a class="moz-txt-link-freetext" href="http://phenix-online.org/presentations/latest/2012_afonine_ecm27-final.pdf">http://phenix-online.org/presentations/latest/2012_afonine_ecm27-final.pdf</a><br>
<br>
and also here<br>
<br>
<a class="moz-txt-link-freetext" href="http://journals.iucr.org/d/issues/2010/11/00/dz5209/index.html">http://journals.iucr.org/d/issues/2010/11/00/dz5209/index.html</a><br>
<br>
Or simply<br>
<br>
- get data and model from PDB<br>
phenix.fetch_pdb pdb_code --mtz<br>
<br>
- compute maps<br>
phenix.maps model.pdb data.mtz <br>
or<br>
phenix.maps model.pdb data.mtz <br>
if it is neutron data set. You may want to remove H so that you can
see them in residual maps:<br>
phenix.reduce -trim model_with_h.pdb > model_no_h.pdb<br>
<br>
- use graphical program of your choice to make a picture (I use
Pymol).<br>
<br>
Examples above use Phenix command line. All the same can be done
using the GUI.<br>
<br>
Pavel<br>
<br>
<br>
<div class="moz-cite-prefix">On 4/17/15 2:58 PM, mohamed noor wrote:<br>
</div>
<blockquote
cite="mid:CAOjUuU=N+iU9_LB_ZwckFJBRyGFchgv_p0X32XjgCEHe15jwag@mail.gmail.com"
type="cite">
<div dir="ltr">
<div>Dear all<br>
<br>
</div>
I will be presenting about protein crystallography to a group of
PhD-level physicists and need some images from the community.<br>
<div><br>
Would anyone have high-quality images of density maps that
show hydrogen atoms at<br>
1. high and ultra-high X-ray resolution (say, about 1.2 A to
about 0.5 A)<br>
2. low or medium X-ray resolution (where H atoms are not
visible) compared with neutron data (with H). Ideally these
images should be from the same protein so that I can show the
power of complementary beam source for the diffraction.<br>
<br>
</div>
<div>A side question - what is the community's preference to get
deuterated samples? Is it crystal soaking or growing E. coli
in deuterium?<br>
</div>
<div><br>
</div>
<div>All contributions will be credited. Thanks.<br>
</div>
</div>
<br>
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<br>
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