<div>i am using 1.7.1, but i will update it to 1.7.3.</div>
<div>i am not sure if the complex has both proteins. Interestingly i am observing this pseudotranslation only in complex structures so i assume that this might be a protein complex with both the proteins. <br><br></div>
<div class="gmail_quote">On Tue, Feb 28, 2012 at 12:13 AM, Nathaniel Echols <span dir="ltr"><<a href="mailto:nechols@lbl.gov">nechols@lbl.gov</a>></span> wrote:<br>
<blockquote style="BORDER-LEFT:#ccc 1px solid;MARGIN:0px 0px 0px 0.8ex;PADDING-LEFT:1ex" class="gmail_quote">
<div class="im">On Sun, Feb 26, 2012 at 10:45 PM, intekhab alam <<a href="mailto:faisaldbg@gmail.com">faisaldbg@gmail.com</a>> wrote:<br>> I have collected 2 datasets; one for the native proteins (2.3Å resolution)<br>
> while other is for a protein-protein complex (3.5Å resolution). The unit<br>> cell parameters for the native are a=120, b=196, c=109, a=g=90, b=114, while<br>> for the complex data it is a=122, b=197, c=300, a=g=90, b=93. I have<br>
> successfully got a MR solution and refined the native dataset with 3<br>> monomers in the asymmetric unit. Analysis of the protein complex dataset<br>> shows 41% pseudo translation and so far there is no success in molecular<br>
> replacement for this dataset. Can anyone please suggest me ways to solve<br>> this problem. i already have tried different templates like monomer, dimer,<br>> trimer, tetramer etc with truncations of loop region. i am using phaser,<br>
> autoMR, molrep.<br><br></div>Which version are you using? 1.7.3 introduced support for TNCS in<br>Phaser but not AutoMR, but the latest nightly builds have this turned<br>on by default so both programs should be able to handle the problem.<br>
(With the minor caveat that the Phaser GUI is broken in dev-991, but<br>hopefully a new build will be available very soon.)<br><br>Aside from TNCS, are you certain that your complex really contains<br>both proteins?<br><br>
-Nat<br>_______________________________________________<br>phenixbb mailing list<br><a href="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a><br><a href="http://phenix-online.org/mailman/listinfo/phenixbb" target="_blank">http://phenix-online.org/mailman/listinfo/phenixbb</a><br>
</blockquote></div><br><br clear="all"><br>-- <br>INTEKHAB ALAM<br>LABORATORY OF STRUCTURAL BIOINFORMATICS<br>KOREA UNIVERSITY, SEOUL<br>