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<p class="MsoNormal">Hi everyone,</p>
<p class="MsoNormal">I have got a 4.2A SeMet MAD dataset for a protein-DNA
complex. <span style>�</span>And, the native
dataset for this protein-DNA complex is ~3.2A. The space group for the native
and SeMet crystals seem to be different.<span style>�
</span>I am not sure whether it�s possible to solve the structure with the
current data I have, and wondering whether any of you have experience with
working with this low-resolution data and any suggestion for me. </p>
<p class="MsoNormal">I processed the data with XDS in space group P31, and used
*.cns.hkl file from XDSCONV as an input for AutoSol and also include
the sequence for protein only. <span style>�</span>The
protein dimer has 418 residues and DNA is 32bp. I run Autosol with the default
setting. Below is statistics I got. It seems that I didn�t get anything
promising. Any comment or suggestion about what to try next? Thank you so much!
</p>
<p class="MsoNormal">Statistics:<span style>� </span></p>
<p class="MsoNormal">Top solution: 2<span style>���������� </span>Sites:
11.<span style>��������������������
</span>Space group: P32.<span style>����������� </span>FOM:
0.550.<span style>������� </span></p>
<p class="MsoNormal">BAYES-CC: 8.10.<span style>��������� </span>Residues:
465<span style>����������
</span>Side-chains: 0.<span style>����� </span><span style>������������</span>Chains:
60. </p>
<p class="MsoNormal">Model CC:<span style>�
</span>0.67<span style>����������
</span>R-work: 0.4100<span style>������� </span><span style>��</span>R-free: 0.4569. </p>
<p class="MsoNormal">Under Heavy-atom search and phasing:</p>
<table class="MsoTableGrid" style="border-collapse:collapse;border:none" border="1" cellpadding="0" cellspacing="0">
<tbody><tr style>
<td style="width:60.15pt;border:solid black;border:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="60">
<p class="MsoNormal">�</p>
</td>
<td style="width:52.65pt;border:solid black;border:1.0pt;border-left:none;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">Space group</p>
</td>
<td style="width:52.95pt;border:solid black;border:1.0pt;border-left:none;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal"># of refined sites</p>
</td>
<td style="width:52.6pt;border:solid black;border:1.0pt;border-left:none;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">FOM</p>
</td>
<td style="width:53.1pt;border:solid black;border:1.0pt;border-left:none;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">Overall score</p>
</td>
<td style="width:51.45pt;border:solid black;border:1.0pt;border-left:none;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="51">
<p class="MsoNormal">R-factor</p>
</td>
<td style="width:51.45pt;border:solid black;border:1.0pt;border-left:none;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="51">
<p class="MsoNormal">Map skew</p>
</td>
<td style="width:51.45pt;border:solid black;border:1.0pt;border-left:none;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="51">
<p class="MsoNormal">Corr.<span style>� </span>of
local RMS density</p>
</td>
</tr>
<tr style>
<td style="width:60.15pt;border:solid black;border:1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="60">
<p class="MsoNormal">Solution1</p>
</td>
<td style="width:52.65pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">P31</p>
</td>
<td style="width:52.95pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">11</p>
</td>
<td style="width:52.6pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">0.530</p>
</td>
<td style="width:53.1pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">8.10+/-11.80</p>
</td>
<td style="width:51.45pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="51">
<p class="MsoNormal">0.4184</p>
</td>
<td style="width:51.45pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="51">
<p class="MsoNormal">-0.12</p>
</td>
<td style="width:51.45pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="51">
<p class="MsoNormal">0.66</p>
</td>
</tr>
<tr style>
<td style="width:60.15pt;border:solid black;border:1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="60">
<p class="MsoNormal">Solution2</p>
</td>
<td style="width:52.65pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">P32</p>
</td>
<td style="width:52.95pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">11</p>
</td>
<td style="width:52.6pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">0.550</p>
</td>
<td style="width:53.1pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="53">
<p class="MsoNormal">8.10+/-11.80</p>
</td>
<td style="width:51.45pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="51">
<p class="MsoNormal">0.3881</p>
</td>
<td style="width:51.45pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="51">
<p class="MsoNormal">-0.10</p>
</td>
<td style="width:51.45pt;border-top:none;border-left:none;border-bottom:solid black;border-bottom:1.0pt;border-right:solid black;border-right:1.0pt;padding:0cm 5.4pt 0cm 5.4pt" valign="top" width="51">
<p class="MsoNormal">0.66</p>
</td>
</tr>
</tbody></table>
<p class="MsoNormal">�</p><p class="MsoNormal">Best,<br>Wei<br></p>