<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div><span>Dear All,</span></div><div><span></span> </div><div><span>After we refine the structure of the protein to satisfactory with satisfactory Rwork and Rfree, we pick water by phenix refine, and I find <var id="yui-ie-cursor"></var>Rfree always increases slightly after the water picking refinment. </span></div><div><span></span> </div><div><span>Do you have nay idea to solve this problem or any comment?</span></div><div><span></span> </div><div><span>Cheers,</span></div><div><span></span> </div><div><span>Dialing</span></div><div><br></div> <div style="font-family: times new roman, new york, times, serif; font-size: 12pt;"> <div style="font-family: times new roman, new york, times, serif; font-size: 12pt;"> <div dir="ltr"> <font size="2" face="Arial"> <div style="margin: 5px 0px; padding: 0px;
border: 1px solid rgb(204, 204, 204); height: 0px; line-height: 0; font-size: 0px;" class="hr" contentEditable="false" readonly="true"></div> <b><span style="font-weight: bold;">From:</span></b> Nathaniel Echols <nechols@lbl.gov><br> <b><span style="font-weight: bold;">To:</span></b> PHENIX user mailing list <phenixbb@phenix-online.org> <br> <b><span style="font-weight: bold;">Sent:</span></b> Tuesday, 7 February 2012 2:03 PM<br> <b><span style="font-weight: bold;">Subject:</span></b> Re: [phenixbb] on Rwork and Rfree<br> </font> </div> <br>On Mon, Feb 6, 2012 at 7:57 PM, Dialing Pretty <<a href="mailto:hdc123hdc123@yahoo.com" ymailto="mailto:hdc123hdc123@yahoo.com">hdc123hdc123@yahoo.com</a>> wrote:<br>> But how about if both the Rfree and Rwork decrease, by comparing the start<br>> and final, however in the middle of the the refinement (for example the<br>> second cycle of the default3 cycles) the Rfree and Rwork are higher
in<br>> comparison with the Rfree_start and Rwork_statrt?<br><br>This is okay, and not at all uncommon (especially if you use simulated<br>annealing).<br><br>-Nat<br>_______________________________________________<br>phenixbb mailing list<br><a href="mailto:phenixbb@phenix-online.org" ymailto="mailto:phenixbb@phenix-online.org">phenixbb@phenix-online.org</a><br><a href="http://phenix-online.org/mailman/listinfo/phenixbb" target="_blank">http://phenix-online.org/mailman/listinfo/phenixbb</a><br><br><br> </div> </div> </div></body></html>