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Hi Dialing,<br>
<br>
<blockquote
cite="mid:1328674974.68757.YahooMailNeo@web121702.mail.ne1.yahoo.com"
type="cite">
<div style="color:#000; background-color:#fff; font-family:times
new roman, new york, times, serif;font-size:12pt">When we run 3
rounds of Phenix refine, both the Rwork and Rfree are
satisfactory. However if we run 5 rounds of Phenix refine with
the same strategy as the above 3 rounds, in the 4th round Rfree
starts to increase until the end of the 5th round.<br>
<br>
Although by the above 3 rounds of Phenix refine we got
satisfactory Rwork and Rfree, can we say the results got by that
3 rounds of Phenix refine are acceptable?<br>
</div>
</blockquote>
<br>
it's nearly impossible to tell what's going on given the amount of
information you provided. In this case it will be much more
efficient if you send me the input data and model files, and I will
send you back working example with the best refinement strategy, and
explain it. I will also post the summary to phenixbb.<br>
<br>
Pavel<br>
<br>
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