[phenixbb] Problems with sharpening in Autosharpen

[Carlos HENRIQUE FERNANDES]

Dear all, 

I am facing some problems with sharpening in Autosharpen. 
My current map is about 3.2 Å resolution, which I obtained after CTF-Refinement and Polishing in Relion. 
When I use Autosharpen in default parameters, it tells me that are applied an overall Bsharp applied of 220.34 and a final Biso of 87.42; but very little sharpening is done; I was unable to observe a typical helices sharpening at this value of resolution. 
I did some tests setting progressive Bsharp values (240; 260; 280; 300), but I am still unable to visualize a typical helices sharpening. 
Has anyone had problems like that? Any tips? 
Thanks in advance, 
Carlos. 
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://phenix-online.org/pipermail/phenixbb/attachments/20210521/eefd6a28/attachment.htm>