[phenixbb] high B Factor after real space refinement against a cryoEM map

Keda Zhou Keda.Zhou at colorado.edu
Tue Oct 15 15:04:00 PDT 2019

Dear all,
I am trying to do the real space refinement for my model against a 5.0 angstrom cryoEM map. All parameters look pretty good except the B factor. The mean of the B factor is close to 400. I am just wondering whether the low resolution makes the B factor this high.
Does anyone have any idea or suggestion on the high B factor during real space refinement? Is it possible to reduce it? What is the actual meaning of this B factor?
Thanks in advance.
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