[phenixbb] Base-pair restraints and high resolution cryoEM maps

Stephen Cusack cusack at embl.fr
Mon Oct 7 09:47:36 PDT 2019

Dear All,

(1) Is it possible to add RNA base-pair restraints into Phenix 
Real-space-refinement of a lowish resolution cryoEM structure?

If so how?

(2) We are also lucky enough to have some impressively high resolution 
cryoEM maps (2.4 A average) which show among other things hundreds of 
water molecules.

I was wondering whether Phenix Real-space-refinement was really adapted 
to high resolution, e.g. it uses grouped B-factor refinement and has no 
water handling options

(putting them in manually is a pain and you always miss some). How have 
other people dealt with these issues ?

thanks for any help

Stephen Cusack


Dr. Stephen Cusack, FRS
Head of EMBL Grenoble
Group leader in the structural biology of protein-RNA complexes and viral proteins

Email:	cusack at embl.fr, stephen.cusack at embl.org			
Website: http://www.embl.fr			
Tel:	(33) 4 76 20 7238    Secretary (33) 4 76 20 7123				
Fax:    (33) 4 76 20 7199					
Postal address:   EMBL Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
Delivery address: EMBL Grenoble Outstation, Polygone Scientifique, 71 Avenue des Martyrs, 38000 Grenoble, France

More information about the phenixbb mailing list