[phenixbb] Real space refine of long side chains

Luca Pellegrini lp212 at cam.ac.uk
Mon Nov 25 11:11:16 PST 2019

I have noticed that real-space refinement in cryoEM maps has a tendency to pull long disordered side chains (Lys, Glu etc) back towards main chain density, resulting in unrealistic hairpin-like bent conformations. Is there a way to counter or moderate this behaviour? It would save me from having to manually straighten them all out again afterwards...

Best wishes,

Luca Pellegrini, PhD
Department of Biochemistry
University of Cambridge
Cambridge CB2 1GA
United Kingdom

lp212 at cam.ac.uk
tel.: 0044 1223 760469

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