[phenixbb] Secondary structure restraints

Luca Pellegrini lp212 at cam.ac.uk
Wed Nov 20 11:03:51 PST 2019

I have a protein-DNA complex and would like to apply SS restraints. What is the best way to apply the SS restraints to the protein only, using the GUI? I have tried the following: 
Select ‘Use secondary structure restraints’ in the ‘Refinement Settings’ tab
‘Select Atoms’
In the Selection Editor window, 'Find Secondary Structures'
Removed manually all nucleic acid restraints in ‘Base Pairs’ and Stacking Pairs’ tabs
Save and close.

However, phenix refinement keeps switching SS restraints for nucleic acids to True.

Kind regards,

Luca Pellegrini, PhD
Department of Biochemistry
University of Cambridge
Cambridge CB2 1GA
United Kingdom

lp212 at cam.ac.uk
tel.: 0044 1223 760469

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