[phenixbb] Local sharpening error

[Ricardo Righetto]

OK Tom, thanks a lot for the very quick reply! I'll try that.

But now I'd like to understand - why is the sequence file necessary at all?
I thought the local sharpening approach just did the same as the global
sharpening, only sliding a small box throughout the volume. It seems then
that this is not the case?

Best wishes,


--
Ricardo Diogo Righetto

2018-01-18 21:53 GMT+01:00 Tom Terwilliger <
tterwilliger at newmexicoconsortium.org>:

> Hi Ricardo,
>
> I'm sorry, yes you do have to supply a sequence file for local
> sharpening.  I will fix the documentation.  If you want you can just put in
> a dummy sequence and supply a value for "solvent_content=xxx" and that will
> accomplish the same thing.
>
> Let me know if that doesn't do it!
> all the best,
> Tom T
>
> On Thu, Jan 18, 2018 at 1:42 PM, Ricardo Righetto <
> ricardorighetto at gmail.com> wrote:
>
>> Hi,
>>
>> I am trying to do local sharpening with phenix.auto_sharpen, with the
>> following command:
>>
>> phenix.auto_sharpen map.mrc resolution=4.0 local_sharpening=True
>>
>> Then it runs the global sharpening first as expected, but when it gets to
>> the local sharpening it crashes with the following error:
>>
>> Sorry: Please specify a sequence file with seq_file=myseq.seq
>>
>> Nowhere it says that I would need a .seq file in order to do local
>> sharpening (global sharpening works fine). Any ideas?
>>
>> Thanks,
>>
>> --
>> Ricardo Diogo Righetto
>>
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>
>
>
> --
> Thomas C Terwilliger
> Laboratory Fellow, Los Alamos National Laboratory
> Senior Scientist, New Mexico Consortium
> 100 Entrada Dr, Los Alamos, NM 87544
> <https://maps.google.com/?q=100+Entrada+Dr,+Los+Alamos,+NM+87544&entry=gmail&source=g>
> Email: tterwilliger at newmexicoconsortium.org
> Tel: 505-431-0033
>
>
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