[phenixbb] on determination whether a protein is a monomer or complex by phenix

[Keller, Jacob]

Depending on your data quality, you could just put in the expected sequences and see what autosol or phaser outputs.

JPK

From: phenixbb-bounces at phenix-online.org [mailto:phenixbb-bounces at phenix-online.org] On Behalf Of Smith Liu
Sent: Monday, July 06, 2015 7:21 AM
To: Tim Gruene
Cc: phenixbb at phenix-online.org
Subject: Re: [phenixbb] on determination whether a protein is a monomer or complex by phenix

Dear  Tim,

I mean based on the mtz file, can I know whether I will get a PDB file of a monomer or dimer, or trimer, or tetramer or oligomer?

Best regards.

Smith




At 2015-07-06 18:14:03, "Tim Gruene" <tg at shelx.uni-ac.gwdg.de<mailto:tg at shelx.uni-ac.gwdg.de>> wrote:

>Dear Smith,

>

>I would say that size exclusion chromatography or native PAGE are better

>experimental techniques than crystallography for this type of question.

>

>Best regards,

>Tim

>

>On 07/06/2015 11:20 AM, Smith Liu wrote:

>> Dear All,

>>

>>

>> Suppose I have got a mtz file, and I know the aa sequence of the protein from which I got the crystal and the corresponding mtz file, will you please tell me which phenix function (program) can tell me whether my protein crystal was formed by protein monomer or by protein complex, and if formed by protein complex, how many subunits existing in the complex?

>>

>>

>> Best regards.

>>

>>

>> Smith

>>

>>

>>

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>--

>--

>Dr Tim Gruene

>Institut fuer anorganische Chemie

>Tammannstr. 4

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>phone: +49 (0)551 39 22149

>

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>

>

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