[phenixbb] Phasing using CryoEM Map
Pavel Afonine
pafonine at lbl.gov
Mon Sep 1 22:32:57 PDT 2014
Hi Alex,
> I trying to phase my 4Å data with low resolution cryoEM maps (various
> 12-18Å). There is some documentation, but not really for dealing with
> EM data. I am having trouble converting the EM data from mrc to
> suitable F's for phaser.
isn't it a matter of Fourier transforming your map into structure
factors and then using them down the road pretending that these are your
"Fobs" and however obtained phases?
If that's the case then phenix.map_to_structure_factors is the tool you
need:
http://www.phenix-online.org/documentation/reference/map_to_structure_factors.html
If not I wonder what am I missing?
Pavel
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