[phenixbb] anomalous signal

Tim Gruene tg at shelx.uni-ac.gwdg.de
Mon Oct 20 10:10:22 PDT 2014


Hi Matteo,

you are right, the absolute value contains the contribution from all
absorbing elements. The edge can be determined reliably, though, because
it is not shifted with respect to the energy (by this effect), only with
respect to the amplitude.

Best,
Tim

On 10/20/2014 02:44 PM, Matteo Colombo wrote:
> Hello,
> I was wondering if the f'' determined by XANES experiment is
> quantitatively significant and if it can be contributed by two different
> anomalous scatterers.
> 
> For example in my protein I know that Co and Zn are present. In theory,
> at Zn absorption edge, I have a contribution from Co too. If I perform a
> XANES experiment to determine Zn absorption edge and I obtain a f'' of
> 5.87, does this value correspond to only Zn signal or to a sum of Zn+Co?
> 
> Thanks a lot
> 
> Matteo
> 

-- 
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

GPG Key ID = A46BEE1A

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