[phenixbb] scaling in phenix.fobs_minus_fobs_map

[Pavel Afonine]

Hi Michael,

phenix.fobs_minus_fobs_map will match two Fobs data sets, scale them 
together and compute fo1-fo2 map coefficients.
No need to scale them before supplying to phenix.fobs_minus_fobs_map, 
also you don't need to truncate one of them to match high resolution.

Pavel

On 2/25/14, 2:02 PM, Michael Thompson wrote:
> Hello,
>
> I have a very simple question about phenix.fobs_minus_fobs_map, and it's implementation in the GUI. I just want to make sure I am not skipping an important step in generating my isomorphous difference map. I'm trying to create an Fo-Fo map for two data sets at pretty different resolutions, using phases from a model. I truncated the higher-res data so the high resolution limit matches the lower-res data set. Are these 2 mtz files sufficient for input into phenix.fobs_minus_fobs_map, or do I need to use another utility to scale the two data sets together prior to calculating the map coefficients? I suppose another way to ask my question would be, does phenix.fobs_minus_fobs_map perform scaling of the two Fobs arrays before calculating the new map?
>
> Thanks for clarifying,
>
> Mike
>
>
>
>
>
>
>