[phenixbb] few questions about resolving new structure through MR

[Zhihong Yu]

Dear  Nathaniel,

Thanks for your reply. So from your answer, I feel that maybe it's not
worth to spend much time to resolve the structure through MR based on
current dataset? Even I can correctly place the domainB, it's still hard to
resolve the intact protein structure without other phasing information, is
that right?

Thanks again and best regards!

Zhihong


>
>>
>
> I confess I haven't had to solve a structure like this myself - just seen
> a bunch of datasets where similar attempts didn't work - but it's hard to
> see how it can be solved without experimental phasing information (SeMet,
> etc.).  The good news is that if you can get a correct MR solution for
> domainB, you can use MR-SAD to find heavy atom sites and solve the
> structure, which should be easier than starting from no phases at all.
>
> -Nat
>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://phenix-online.org/pipermail/phenixbb/attachments/20131107/8035ba5a/attachment-0001.htm>