[phenixbb] TLS makes my r-factors shoot through the roof
Morten Groftehauge
mortengroftehauge.work at gmail.com
Tue May 29 03:56:22 PDT 2012
Hi Phenixbb,
3.2A data, 110 Wilson B, I41, twinned. Imperfect inverted repeat dsDNA with
4 protein monomers, NCS not used in refinement.
If I don't include TLS I get a nice stable R-factor while RMS goes down.
If I include TLS (whether as one group per chain or as chosen by Phenix)
this happens:
[image: Inline images 1]
Density disappears around some of the DNA bases as an obvious difference.
If I run Refmac with TLS it just never finishes.
While I could just refine without TLS this does make me worried that there
is something wrong with my model or my data (besides being terrible).
Cheers,
Morten
--
Morten K Grøftehauge, PhD
Pohl Group
Durham University
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://phenix-online.org/pipermail/phenixbb/attachments/20120529/13be139a/attachment-0001.htm>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: not available
Type: image/png
Size: 27991 bytes
Desc: not available
URL: <http://phenix-online.org/pipermail/phenixbb/attachments/20120529/13be139a/attachment-0001.png>
More information about the phenixbb
mailing list