[phenixbb] problem about phase caculate

yzhao zhaoy at moon.ibp.ac.cn
Tue Dec 18 16:04:06 PST 2012

Hi everyone,
I have came into a problem recently. My structure was solved by MAD and a crude
model was built manually.
Several peak data sets were collected, resolution is about 4A-4.2A. The heavy
atom can be located by anomalous difference fourier. I feed the initial phase
or heavy atom sites, and the peak data set to phenix. However the map after DM
is too bad. The density is very strange. I don't know what's the problem?

In addition, how to define protein mask at phenix autosol for density

Thanks in advance.


Yan Zhao, M.Phil.
National Laboratory of Protein Sciences 
Institute of Biophysics 
Chinese Academy of Sciences 
15 Datun Rd.
Beijing, 100101 
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