[phenixbb] fwd: Re: pseudotranslation and twinning problem

Nathaniel Echols nechols at lbl.gov
Mon Dec 3 13:02:12 PST 2012

(See original reply below.)

---------- Forwarded message ----------
From: Bob Rose <rbrose2 at ncsu.edu>
To: PHENIX user mailing list <phenixbb at phenix-online.org>
Date: Mon, 3 Dec 2012 09:14:21 -0500
Subject: Re: [phenixbb] pseudotranslation and twinning problem
Hi Raj,

You may have the space group wrong. You could try a higher symmetry
space group. If your dataset is complete, see if the data statistics
look almost as good in a higher symmetry space group. You can always
try lower symmetry too. Maybe you are not I-centered.

Good luck,

On Sun, Dec 2, 2012 at 2:32 PM, Rajeshwer Sankhala
<Rajeshwer.Sankhala at jefferson.edu> wrote:
> I am new to crystallography. I have collected data for 26 kDa protein with a
> maximum resolution of 2.25A in I 41 space group. I have a poor model for
> molecular replacement (~15-20% identical). When i did xtriage run, it
> suggested -h,k,-l twinning law and indicated about pseudo-translation. Now
> when i am running Phaser for molecular replacement i am getting very low LLG
> (less than 100) and TFZ score (5-6). Can anyone suggest which parameters or
> setting i should use to improve my LLG score and get a best model for
> refinement.

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