[phenixbb] difficult phasing problem

[Ian Slaymaker]

I am trying to solve the structure of a ~30kd monomeric protein for which
we have 2.5A diffraction data, and a search model with ~65 % similarity (i
have tried, homology models of this and others, as well as using sculpter
to build several variations of core search models). I have tried all my
possible space groups and a huge range of parameters, and mr_rosetta to
rebuild some of my better (but still bad) MR solutions. My LLG
is persistently negative (<-1000), which may be due to translational
noncrystallographic symmetry which was identified by xtriage. We have
unfortunatly been unable to phase this data with selmet SAD, so I am
looking for some other MR things to try. Any suggestions for working with
tNCS (if this is really the problem) would be much appreciated.
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