[phenixbb] Geometry Restraints - Anisotropic truncation

[Leonid Sazanov]

Hi, so will we see now the possibility to apply anisotropic resolution 
limits truncation in Phenix?
Despite the fact that a lot of people use UCLA server, there is still no 
possibility to do this in any major crystallographic software, AFAIK.
Phaser does the scaling, but no truncation, and noise sitting in those 
regions where there is no signal, is a real culprit.
Large SIGFs there do not resolve the problem completely.
Thanks.


 >>>>>>
Message: 4
Date: Wed, 25 Apr 2012 09:05:57 -0400
From: Mario Sanches <mariosan at gmail.com>
To: PHENIX user mailing list <phenixbb at phenix-online.org>
Subject: [phenixbb] Geometry Restraints - UPDATE
..........
It turns out that the reason to my nightmares was a highly anisotropic
dataset. When I tried to fix geometry related problems in coot,
phenix.refine was twisting the geometry back again, probably because it was
trying to fit a lot of noise due to the anisotropy. Everything was getting
worst, geometry, clashscore, and Ramachandran.

Pavel finally corrected it by using this server:
http://services.mbi.ucla.edu/anisoscale/

............


-- 
Dr. Leonid A. Sazanov
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Medical Research Council
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