[phenixbb] is derivative data useful for looking for the missing part of the model?
Francis E Reyes
Francis.Reyes at Colorado.EDU
Tue Jun 15 16:03:07 PDT 2010
> . My question is that if refine the model against the derivative
> data, will the heavy atom appear in the difference map if existing?
Using the model to find the heavy atom sites and then calculating
phases on those sites (without model) might help. ( I believe phenix
will output a phase combined map (model + experimental phases)).
Multi crystal averaging might help.
However, as you come from MR, you should try a prime and switch map on
the original data.
On Jun 15, 2010, at 4:59 PM, fn1 at rice.edu wrote:
> Hi everyone,
> I got a partial model, consisting of 80% of the whole molecule, from
> the molecular replacement. The native data reflects to 2.3 A, and
> now the R/Rfree= 0.22/0.28. My problem is that the missing part of
> the model is important for us, but there is not any density for this
> region now.
> Meantime, we have several heavy atom derivative data. My question is
> that if refine the model against the derivative data, will the heavy
> atom appear in the difference map if existing? Any suggestions on
> how to use the derivative data to locate the missing part of model?
> Thanks in advance!
> phenixbb mailing list
> phenixbb at phenix-online.org
Francis Reyes M.Sc.
University of Colorado at Boulder
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