[phenixbb] Double stranded DNA restraints?

[Rajagopalan, Senapathy]

Hi Everyone,

Is there a way to force phenix (autobuild) into building a contiguous double stranded DNA. Currently, when I am solving a protein-DNA complex using SAD data:

1) its not recognizing that DNA needs to be double stranded, so it is fitting one of the strands correctly (at least part of it), but the other strand is in the protein density region.

2) It breaks down each of the strand. So it is not fitting as a contiguous 30 mer but instead a 10 mer, two 6 mers and a 8 mer.

Thanks
Sena


Methodist. Leading Medicine. 

Recognized by U.S.News & World Report as an "Honor Roll" hospital
Named to FORTUNE magazine's "100 Best Companies to Work For" list five years in a row
Designated as a Magnet hospital for excellence in nursing
Visit us at methodisthealth.com
Follow us at twitter.com/MethodistHosp and www.facebook.com/methodisthospital

***CONFIDENTIALITY NOTICE***
This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you.

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://phenix-online.org/pipermail/phenixbb/attachments/20100402/5f92d04c/attachment-0003.htm>