[phenixbb] Autobuild at 5A resolution

Peter Grey petgxray at gmail.com
Wed Sep 16 15:05:09 PDT 2009


 Dear Phenix users,

I am trying to solve a big RNA/protein complex at 5A resolution. Phases come
from MR solution with a partial model in Phaser. Thanks to  NCS the map
after density modification in RESOLVE is of reasonable quality for this
resolution and new secondary structures, out side of the model, can be
detected.
I would like to improve the maps and model with something like a poor man's
version of AutoBuild. An Autobuild for very low resolutions that does not
include refinement  but iteratively carries out density modification and
identification of helices/strands outside of the model with
phenix.find_helices_strand.
Does this sound feasible, Are there already scripts the follow such a scheme
?

Many thanks in advance for your advice


-- 
Peter
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