[phenixbb] Refining f'/f"?
Bryan W. Lepore
Bryan.Lepore at umassmed.edu
Thu May 7 12:26:54 PDT 2009
On Thu, 7 May 2009, Nathaniel Echols wrote:
> I think it's very common for these to have negative difference map
> peaks, because they'll undergo radiation damage very quickly relative to
> the rest of the protein.
my understanding is that if there was significant absorption, and if the
model scattering is uncorrected for f' - which is in-phase with normal
scattering f for that atom - Fc will overestimate Fo, thus, Fo-Fc will be
the f'' component will be -(pi/2) out of phase with the normal f, and -
here is what i don't get - shift the F so its out of phase. but i don't
intutitvely understand how that affects the Fo-Fc map.
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