[phenixbb] Best first map for RESOLVE after MR - PS
Pavel Afonine
PAfonine at lbl.gov
Mon Aug 31 00:05:47 PDT 2009
PS: Make sure you use not fobs-filled map (xxx_no_fill).
Pavel.
On 8/30/09 2:32 PM, Peter Grey wrote:
> Hi Pavel,
>
> Thank you very much for your fast answer. I just thought that maybe
> the bulk solvent correction procedure in phenix can really create
> better maps because with such big complexes I must use all low
> resolution data. Of course I can try both options but there are so
> many things to try with this big complex at low resolution that I
> would like to eliminate options where possible.
>
> Many many thanks,
>
> Peter.
>
>
>
> On Sun, Aug 30, 2009 at 7:22 PM, Pavel Afonine <PAfonine at lbl.gov
> <mailto:PAfonine at lbl.gov>> wrote:
>
> Hi Peter,
>
> I guess this my reply will not replace Tom's or Randy's one...
>
> > Should it be the 2mFo-DFc map directly from Phaser or would it be
> > better to do rigid body refinement in phenix.refine and use the bulk
> > solvent corrected 2mFo-DFc map ?
>
> - why don't you try using both options and choose the best outcome?
>
> - running a round of rigid body refinement in phenix.refine and using
> its 2mFo-DFc map will not hurt at least or may make your model and
> therefore the map better, so you will have a better starting point.
>
> Pavel.
>
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